TFN-HM101-100μg / 询价
TFN-HM101-500μg / 询价
TFN-HM101-500μgx2 / 询价
Human Transferrin Protein
Recombinant Human Transferrin Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val20-Pro698 [Accession | AAH59367].
The protein has a predicted MW of 76.3 kDa. Due to glycosylation, the protein migrates to 78-82 kDa based on Bis-Tris PAGE result.
> 95% as determined by Bis-Tris PAGE
> 95% as determined by HPLC
Less than 1EU per μg by the LAL method.
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
-20 to -80°C for 12 months as supplied from date of receipt.
-80°C for 3 months after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Transferrin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Transferrin is greater than 95% as determined by SEC-HPLC.
Immobilized Human Transferrin, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 0.50μg/ml determined by ELISA (QC Test).
Human Transferrin, His Tag immobilized on CM5 Chip can bind Human Transferrin R, His Tag with an affinity constant of 11.26 nM as determined in SPR assay (Biacore T200).
Transferrin (Tf), an iron transporter, is mainly biosynthesized in the liver, but can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, a cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting β1,4-GlcNAc and core α1,6-Fuc. The glycans of serum-type Tf in the CSF are similar to those of Tf in serum.
Serotransferrin; Transferrin; Siderophilin; TF; PRO1557; PRO2086; HEL-S-71p; TFQTL1
(1) Murakami Y, Saito K, Ito H, Hashimoto Y. Transferrin isoforms in cerebrospinal fluid and their relation to neurological diseases. Proc Jpn Acad Ser B Phys Biol Sci. 2019;95(5):198-210. doi: 10.2183/pjab.95.015. PMID: 31080188; PMCID: PMC6742728.
